Syllabus for Separation and Mass Spectrometry
Separation och masspektrometri
A revised version of the syllabus is available.
- 15 credits
- Course code: 1KB153
- Education cycle: Second cycle
Main field(s) of study and in-depth level:
- Grading system: Fail (U), Pass (3), Pass with credit (4), Pass with distinction (5)
- Established: 2008-03-13
- Established by:
- Revised: 2018-08-30
- Revised by: The Faculty Board of Science and Technology
- Applies from: Spring 2019
120 credits with 60 credits in chemistry including Analytical Chemistry, 10 credits. Proficiency in English equivalent to the Swedish upper secondary course English 6.
- Responsible department: Department of Chemistry - BMC
On completion of the course, the student should be able to:
- describe theoretical models for separation, optimisation and detection for chromatographic (liquid - and gas chromatography) and capillary electrophoretic methods and predict how changes in experimental conditions influence separation with these methods
- describe the principle and the design of the components included in separation and mass spectrometric instrumentation
- account for which type of information that can be obtained from a chromatogram, electropherogram and mass spectrum and carry out evaluation of simple spectra
- choose appropriate technique regarding both separation and detection and choose and justify the choice of instrumentation based on the performance and requirements demanded by the applications
- plan and carry out experiments that permit validation of the performance of methods such as sensitivity and selectivity
- plan and carry out both qualitative and quantitative analysis of for example bioanalytical nature by means of the above mentioned methods
Liquid chromatography (reversed phase, normal phase, ion pair, ion chromatography, size-exclusion), gas chromatography and capillary electrophoresis (capillary zone electrophoresis, capillary electrochromatography, capillary gel electrophoresis). Theoretical models for separation, optimisation, and detection. Instrumentation for chromatography and capillary electrophoresis. Overview of systems to generate and measure vacuum. Sample introduction, ion sources and ionisation principles (electron ionisation, chemical ionisation, laser-induced desorption, chemical and photon ionisation at atmospheric pressure and electrospray). Overview of mass analysers mainly quadrupole, ion trap, time-of-flight and hybrid instruments. Detectors. Information that can be received from a chromatogram, electropherogram and mass spectrum. General aspects of interpretation of spectra generated with electron ionisation, chemical ionisation, electrospray and laser desorption. Connection of separation methods (gas chromatography, liquid chromatography and capillary electrophoresis) to mass spectrometry. Data management. Strategies for both relative and absolute quantification. Analytical applications based on chromatographic and capillary electrophoretic methods.
Laboratory projects elucidating gas - and liquid chromatography and capillary electrophoresis and laboratory work with mass spectrometry coupled to separation methods. Oral and written presentation of results.
The teaching is given as lectures, lessons, seminars and laboratory work. Course introduction, seminars and laboratory work are compulsory parts.
Written examination during the course (4.5 credits) and at the end of the course (4.5 credits). For passed grade on the course, it is required that the laboratory work has been presented and accepted. Laboratory work corresponds to 6 credits. The final grade corresponds to a weighted average of the results of the written examinations and the laboratory work.
If there are special reasons for doing so, an examiner may make an exception from the method of assessment indicated and allow a student to be assessed by another method. An example of special reasons might be a certificate regarding special pedagogical support from the disability coordinator of the university.
- Latest syllabus (applies from Autumn 2023)
- Previous syllabus (applies from Spring 2019)
- Previous syllabus (applies from Spring 2015)
- Previous syllabus (applies from Autumn 2013)
- Previous syllabus (applies from Autumn 2012, version 2)
- Previous syllabus (applies from Autumn 2012, version 1)
- Previous syllabus (applies from Autumn 2011)
- Previous syllabus (applies from Autumn 2010)
- Previous syllabus (applies from Autumn 2009)
- Previous syllabus (applies from Autumn 2008)
Applies from: Autumn 2022
Some titles may be available electronically through the University library.
Gross, Jürgen H.
Mass Spectrometry : A Textbook
3rd ed. 2017.: Cham: Springer International Publishing, 2017
Snyder, Lloyd R.;
Kirkland, Joseph J.;
Dolan, John W.
Introduction to modern liquid chromatography
3. ed.: Oxford: Wiley-Blackwell, cop. 2010
Practical Gas Chromatography [Elektronisk resurs] : A Comprehensive Reference
Berlin, Heidelberg: Springer Berlin Heidelberg, 2014
Reading list revisions
- Latest reading list (applies from Autumn 2022)
- Previous reading list (applies from Spring 2019)