The amine:pyruvate aminotransferase from Vibrio fluvialis JS17, APA, catalyzes the transfer of amino groups from amines and amino acids to α-keto acids, aldehydes and ketones. APA is a PLP-dependent enzyme which shows activity with phenyl-substituted substrates, but poor activity with bulkier amino acceptors. This feature makes it interesting to study APA´s activity with ketone with different substituents and to introduce mutations to increase the activity with larger acceptors and with α-hydroxylated ketones (acyloins).
Our studies on APA aims to…
- establish structure/function relationship of enzyme-catalyzed amino transfer reactions.
- isolate new APA variants which stereoselectively catalyze the amino transfer to bulkier aryl substituted and hydroxylated amino acceptors .