Ongoing research within Lanekoff Group

Nano-DESI for mass spectrometry imaging

Schematic and photograph of nano-DESI,
a mass spectrum of the phospholipid region
and ion images of two phospholipids in brain
tissue.

Nanospray desorption electrospray ionization (nano-DESI) is a liquid extraction technique for localized desorption of molecules from a surface into a continuously flowing solvent, which subsequently transports the molecules to the inlet of the mass spectrometer for electrospray ionization and detection. Ion images showing the distribution of a molecule are created by moving the sample under the nano-DESI probe while continuously acquiring spatially resolved data.

The Lanekoff group works on developments of the nano-DESI setup and modification to the solvent to achieve a quantitative, sensitive, and tailored system to use for molecular mapping in thin tissue sections.

Surface sampling capillary electrophoresis mass spectrometry

Schematic and photograph of SS-CE-MS,
an electropherogram of metabolites in the
grey matter of brain. 

Surface sampling capillary electrophoresis (SS-CE-MS) has been invented and is further being developed in the Lanekoff group. SS-CE-MS is a system for direct sampling of molecules from a biological surface into the separation capillary for subsequent separation, ionization, and mass spectrometric detection. This approach does not require any pretreatment of the sample and enables spatial omics of thin tissue sections.

Molecular characterization with mass spectrometry

MSn spectra from the isomers vaccinic and
oleic acid, optical image and ion image of
their relative distribution in brain.

Molecular characterization with mass spectrometry includes using high mass resolving power with high mass accuracy for annotation. The Lanekoff group extends this approach using MSn fragmentation techniques together with clever chemistry and data modelling for annotating isomers and isobars without time consuming separation.

Single-cell mass spectrometic analysis

Mass spectrum from a single cell, annotation
of selected metabolites and lipids.

Chemical analysis of individual cells is an analytical challenge due to the limited material available but have great benefits for revealing cellular heterogeneity in health and disease. The Lanekoff group is developing ambient techniques for chemical omics analysis of individual cells.

Funding

Our research within the Lanekoff group is funded by the Swedish Research Council (VR), and the Swedish Foundation for Strategic Research (SSF)

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Last modified: 2021-04-20