Ongoing research within Lanekoff Group
Nano-DESI for mass spectrometry imaging
Nanospray desorption electrospray ionization (nano-DESI) is a liquid extraction technique for localized desorption of molecules from a surface into a continuously flowing solvent, which subsequently transports the molecules to the inlet of the mass spectrometer for electrospray ionization and detection. Ion images showing the distribution of a molecule are created by moving the sample under the nano-DESI probe while continuously acquiring spatially resolved data.
The Lanekoff group works on developments of the nano-DESI setup and modification to the solvent to achieve a quantitative, sensitive, and tailored system to use for molecular mapping in thin tissue sections.
Surface sampling capillary electrophoresis mass spectrometry
Surface sampling capillary electrophoresis (SS-CE-MS) has been invented and is further being developed in the Lanekoff group. SS-CE-MS is a system for direct sampling of molecules from a biological surface into the separation capillary for subsequent separation, ionization, and mass spectrometric detection. This approach does not require any pretreatment of the sample and enables spatial omics of thin tissue sections.
Molecular characterization with mass spectrometry
Molecular characterization with mass spectrometry includes using high mass resolving power with high mass accuracy for annotation. The Lanekoff group extends this approach using MSn fragmentation techniques together with clever chemistry and data modelling for annotating isomers and isobars without time consuming separation.
Single-cell mass spectrometic analysis
Chemical analysis of individual cells is an analytical challenge due to the limited material available but have great benefits for revealing cellular heterogeneity in health and disease. The Lanekoff group is developing ambient techniques for chemical omics analysis of individual cells.