Epoxidhydrolas
Epoxidhydrolaset StEH1 (från potatis) har visat sig vara en mycket lovande enzymkatalysator för hydrolys av epoxider. Enzymet är stereoselektivt, lätt att producera, strukturellt stabilt även efter mutagenes och vi har samlat in mycket struktur/aktivitetsdata under de senare åren. Vi studerar detta enzym och laboratorieutvecklade variantenzymer genom en kombination av funktions-, strukturanalyser och beräkningskemiska metoder.
Vår forskning på StEH1 siktar till...
- att förstå struktur/funktionssamband i enzymkatalyserad ringöppning av epoxider.
- isolera nya stereoselektiva enzymer lämpliga som biokatalysatorer.
Publikationer
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Epoxide Hydrolysis as a Model System for Understanding Flux Through a Branched Reaction Scheme
Ingår i IUCrJ, s. 269-282, 2018.
DOI för Epoxide Hydrolysis as a Model System for Understanding Flux Through a Branched Reaction Scheme Ladda ner fulltext (pdf) av Epoxide Hydrolysis as a Model System for Understanding Flux Through a Branched Reaction Scheme
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Conformational Diversity and Enantioconvergence in Potato Epoxide Hydrolase 1
Ingår i Organic and biomolecular chemistry, s. 5639-5651, 2016.
DOI för Conformational Diversity and Enantioconvergence in Potato Epoxide Hydrolase 1 Ladda ner fulltext (pdf) av Conformational Diversity and Enantioconvergence in Potato Epoxide Hydrolase 1
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Laboratory evolved enzymes provide snapshots of the development of enantioconvergence in enzyme-catalyzed epoxide hydrolysis
Ingår i ChemBioChem (Print), s. 1693-1697, 2016.
DOI för Laboratory evolved enzymes provide snapshots of the development of enantioconvergence in enzyme-catalyzed epoxide hydrolysis Ladda ner fulltext (pdf) av Laboratory evolved enzymes provide snapshots of the development of enantioconvergence in enzyme-catalyzed epoxide hydrolysis
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Obtaining optical purity for product diols in enzyme-catalyzed epoxide hydrolysis: contributions from changes in both enantio- and regioselectivity
Ingår i Biochemistry, s. 7627-7637, 2012.
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One-step enzyme extraction and immobilization for biocatalysis applications
Ingår i Biotechnology Journal, s. 463-469, 2011.
DOI för One-step enzyme extraction and immobilization for biocatalysis applications
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Deep eutectic solvents (DESs) are viable cosolvents for enzyme-catalyzed epoxide hydrolysis
Ingår i Journal of Biotechnology, s. 169-171, 2010.
DOI för Deep eutectic solvents (DESs) are viable cosolvents for enzyme-catalyzed epoxide hydrolysis
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Modification of substrate specificity resulted in an epoxide hydrolase with shifted enantiopreference for (2,3-epoxypropyl)benzene
Ingår i ChemBioChem (Print), s. 1422-1429, 2010.
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Mutations in salt-bridging residues at the interface of the core and lid domains of epoxide hydrolase StEH1 affect regioselectivity, protein stability and hysteresis
Ingår i Archives of Biochemistry and Biophysics, s. 165-173, 2010.
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Structure-function relationships of epoxide hydrolases and their potential use in biocatalysis
Ingår i Biochimica et Biophysica Acta, s. 316-326, 2010.
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Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability
Ingår i Protein Science, s. 1275-1284, 2008.
DOI för Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability Ladda ner fulltext (pdf) av Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability
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Active site of epoxide hydrolases revisited: A noncanonical residue in potato StEH1 promotes both formation and breakdown of the alkylenzyme intermediate
Ingår i Biochemistry, s. 2466-2479, 2007.
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X-ray structure of potato epoxide hydrolase sheds light on its substrate specificity
Ingår i Protein Science, s. 1628-1637, 2006.
DOI för X-ray structure of potato epoxide hydrolase sheds light on its substrate specificity
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Catalysis of potato epoxide hydrolase, StEH1
Ingår i Biochemical Journal, s. 633-640, 2005.
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The Saccharomyces cerevisiae ORF YNR064c protein has characteristics of an 'orphaned' epoxide hydrolase
Ingår i Biochimica et Biophysica Acta - Proteins and Proteomics, s. 213-221, 2005.